Regulatory

Part:BBa_K2913022

Designed by: Pengchao Wang   Group: iGEM19_NEFU_China   (2019-10-20)


P-BAD

The P-BAD expression system allows tightly controlled, titratable expression of your protein through the regulation of specific carbon sources such as glucose, glycerol, and arabinose. P-BAD is ideal for expressing toxic proteins and optimizing protein solubility in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 265
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 82


[edit]
Categories
Parameters
None